One Two
You are here: Home Home Research Methods



We use freshly isolated cells and acute brain slices of mice and rats. In addition acute, vital hippocampal specimens obtained from epilepsy surgery are included in our studies on a regular basis.

  • patch clamp technique (whole cell, perforated patch, excised patch modes)
  • rapid application techniques

Molecular biology
  • single cell RT-PCR (multiplex, real time TaqMan)
  • use of transgenic animals
    (e.g. EGFP/GFAP; YFP/NG2; Kir4.1-/-; AQP4 -/-; Cx30-/-/CX43fl/fl:GFAP-Cre etc.)

  • postrecording immunolabeling of cells in brain slices
  • biocytin filling for visualization of dye coupling and electron microscopic inspection

Confocal / Two photon imaging

Microfluometry (Ca2+ imaging)

Photolysis-based uncaging (Ca2+, ATP)
Document Actions